This reduced their combined shareholding from 2.29 per cent to 2.01 per cent, it added.
Earlier this month, another regulatory filing had stated that Natarajan and his family had sold over 42 lakh shares of the company in multiple tranches between April 30 and September 14 that had reduced their combined shareholding to 2.29 per cent.
Krishnakumar Natarajan, who held 1.96 per cent share, sold about 2.85 lakh shares, the filing said on Saturday.
Post the transaction, his shareholding reduced to about 1.79 per cent. Akila sold about 1.32 lakh shares between September 15-21, bringing down her holding from 0.19 per cent to 0.11 per cent.
Siddarth Krishna Kumar sold 49,405 shares and bought 1,000 shares, bringing his latest shareholding to 0.10 per cent.
The shares were sold and purchased in the open market, the filing said.
Larsen and Toubro, which acquired majority share in Mindtree last year, had 61.08 per cent shareholding at the end of June 2020 quarter.